The technique used can be applied to any mole to gram conversion. Just place your order while logged in to your Home Science Tools account and you'll automatically earn up to 6% back when your order ships! The 100 mM EDTA stock solution is made with 1.86 g into 40 ml H 2O and then add NaOH to dissolve and adjust pH to 7.4. Explain why water is called a polar covalent molecule. 11.1 g CaCl 2. MEDICATION (VET): The effects of amrinone and calcium chloride on pulmonary vasculature and biventricular function in sheep with acute lung injury were studied. Break up hard lumps of chemical with a mortar and pestle or by careful crushing in a plastic bag with a hammer. 15. The amount of water needed will be slightly less than 100 ml. Question: 1) To Make A 1M Solution Of CaCl2, How Many Grams Would You Dissolve In 100 Mls? It is really impractical to make an exact 100ml mM solution of CaCl2 directly. Answer: dissolve 0.0111g CaCl2 in 100ml solution and you will have a mM solution. 2) To Make 5M Solution Of KOAc How Many Grams Pf Potassium Acetate Would You Dissolve In 100 Mls? Calcium chloride 0.1 M solution; CAS Number: 10043-52-4; Synonym: Calcium chloride solution, Additive Screening Solution 21/Kit-No 78374; Linear Formula: CaCl2; find Sigma-Aldrich-53704 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Scaling down reduces safety hazards, chemical cost, and waste disposal. This is a mM solution. You might consider scaling down when experiments call for large amounts of chemical. A mole consists of 6.02×1023 molecules or atoms. Determine MW using a periodic table by adding the atomic mass of each atom in the chemical formula. Still have questions? Measure the volume from the curve at the top of the solution, or the meniscus, to get the most accurate reading. Get your answers by asking now. Bake at 350 degrees for 40 minutes. Place the stopper in the flask and gently swirl the flask until all the solute is dissolved. Wash all the solute into the bottom of the flask as you do so. Transfer the solute to a clean, dry 100ml volumetric flask. Molecular weight (MW) is the weight of one mole of a chemical. You prepare a solution by dissolving a known mass of solute (often a solid) into a specific amount of a solvent. Materials 1L stock of 1M CaCl 2 (makes ~3000 kits) 1. Weigh out 18.61 g EDTA disodium salt, dihydrate and add to a 100 mL Duran bottle. CaCl2-solution: Category: Buffers & Solutions. When making chemical solutions, always use the appropriate safety equipment. The Molecular Weight Of CaCl2 Is 110.98 G/mol. My Science Perks is FREE! for 10 minutes at 4ºC. How to make a 100 ml of a solution that is 5 mM MgCl2, 5 mM CaCl2 and 125 mM KCl from 1 M stocks of each of these chemicals. A strip of magnesium weighs 0.8197 g. determine the volume in L if the density of magnesium is 1.74 g/mL? Explain the difference between “suspension and Emulsion” OR between “solution and colloid”. CaCl 2 (0.1 M) . Take 0.07 moles/liter times 342.3 grams per mole and you have 23.96 grams needed per liter. Preparation of CaCl2 solution. Join Yahoo Answers and get 100 points today. You mix them together with 1:1 volume ratio, if the pH is kept at 6.3-7.5, then I am wrong. To prepare a 2.5 M stock solution of CaCl 2, dissolve 11 g of CaCl 2 •6H 2 O in a final volume of 20 mL of H 2 O. Sterilize the solution by passing it … Next, weigh out the solute and add it to a mixing beaker. Add distilled water slowly to the volumetric flask. What is the percent by mass of a solution that contains 26.5 g of glucose in 500 g of solution? Add a magnetic flea and place on a magnetic stirring plate to mix the solution. How to Dissolve Solids and Scale Down Experiments. Take 10ml of this solution and dilute again to 1000ml . The solvent, often water, is the chemical that’s most abundant. You can make scaling down easier by using smaller beakers, test tubes, and other measuring equipment. This SOP sets the safety and procedural specifications for making a 50mM stock solution of calcium chloride. # Add 100 mL solution 1 000 ppm in a volumetric flask. grams of solute = (wt% solution) x (ml of water) ÷ (100 – wt% solution), grams of NaCl = (10) x (100) ÷ (100 – 10) = 11.1 g. Preparation of solutions calculator is a useful tool which allows you to calculate how many solid chemicals or stock solutions you will need to prepare the desired solution. Percent by mass (m/m) is the mass of solute divided by the total mass of the solution, multiplied by 100 %.. for 1L Tris, start with 900ml Add 0.1211g of Tris for each 10ml dH20 It is really impractical to make an exact 100ml mM solution of CaCl2 directly Note: This is a very theoretical answer. that will might help! Materials - CaCl2.2H2O (147.01 mol-1) Experiment Settings - Volume of CaCl2 solution: - Molarity: Step 1. How to Make Weight Percent (Wt%) Solutions. Join our list for the latest on products, promotions, and experiments and receive FREE economy shipping on your first $50+ order. Autoclavable. Spin down at 4000r.p.m. Table 3b: Properties for Calcium Chloride Solutions in Metric Units at 25°C % CaCl 2 Specific Density Liters per Liters per Freeze Point Boiling Point Gravity (kg/liter) 1000 kg Solution 1000 kg Dry (°C) (°C) 0 1 .0000 .9971003 n/a 0 100 1 1 .0091 .006994 99,406 -1 100 2 1 .0181 .015985 49,264 -1 100 The solute is the chemical(s) that’s less abundant. Chemicals dissolve more quickly by gently heating the solution and stirring. Once the molecular weight of the solute is known, the weight of chemical to dissolve in a solution for a molar solution less than 1M is calculated by the formula: For example, to make 100 ml of 0.1 M CaCl2 solution, use the previous formula to find out how much CaCl2 you need: Now you can make your solution: dissolve 1.11 g of CaCl2 in sufficient water to make 100 ml of solution. Author: Admin eLABJournal. Make Sure To Include All Calculations. difficult thing. Materials 2L stock of 50mM CaCl 2 (makes ~165 kits) 1. Grow culture at 37°C in shaker overnight. Question: You Are Told To Make A 10 MM Solution Of CaCl2. There are two types of percent concentration: percent by mass and percent by volume.. LEARN MORE >>, Tips for Teaching Science to Elementary Students (Grades 3-5), ©2017 – 2021 Home Science Tools  All Rights Reserved, Save 10%* on Dissection Supplies with code DISSECT21, Save 10%* on Dissection with code DISSECT21, grams of chemical = (molarity of solution in mole/liter) x (MW of chemical in g/mole) x (ml of solution) ÷ 1000 ml/liter. Weigh out amount of solute needed using a balance. To make 200 milliliters of your solution multiply grams/liter by liters needed. Just add the solute to the graduated cylinder and then add distilled water until you reach the 100ml mark in the side of the cylinder. The amount of water needed will be slightly less than 100 ml. Finally, measure the volume of water needed in milliliters with a graduated cylinder and add it to the beaker. Prepare starter culture of cells Select a single colony of E. coli from fresh LB plate and inoculate a 10 mL starter culture of LB (or your preferred media – no antibiotics). Question: 5. Spin down at 4000r.p.m. Calculate the weight of solute needed to make 100ml of solution using the above formula. Since the density of water is 1 g/ml, the formula to calculate the amount of solute that must be mixed for a weight percent solution is: As an example, to make 100 ml of 10% NaCl (table salt) solution, use the previous formula to find out how much NaCl you need: Now you can make your solution: dissolve 11.1 g NaCl in 100 ml of water. Take 10ml of this solution and dilute again to 1000ml . This SOP sets the safety and procedural specifications for making a 1M stock solution of calcium chloride. Dilute 10x to make 100 mM CaCl2. All solutions contain a solvent and one or more solutes. The dissolution process is exothermic and above 60 °C can be reached rapidly. Objective . Second, calculate the amount of solute needed in grams, using one of the formulas given below. The drug is a small organic compound with a molecular weight of about 140 g/mol. Calcium chloride is an inorganic compound, a salt with the chemical formula CaCl 2.It is a white coloured crystalline solid at room temperature, and it is highly soluble in water. Save 10%* on Dissection with code DISSECT21. Version: 1. Complications With Formula Weights Perhaps you cannot find a formula weight on a label or perhaps you are planning a protocol and do not have the actual chemicals on hand. How to make 0.5M EDTA pH 8.0. A concentration of 70 mM is the same as 0.07 moles per liter. It can be created by neutralising hydrochloric acid with calcium hydroxide.. Calcium chloride is commonly encountered as a hydrated solid with generic formula CaCl 2 (H 2 O) x, where x = 0, 1, 2, 4, and 6. Dissolve 11.1 g CaCl 2 in 1 L H 2 O. Autoclave and store in aliquots at -20°C or 4°C.. CiteULike; Delicious; Digg; Facebook; Google+; Reddit; Twitter; What's this? Your strategy here will be to use the molarity and volume of the solutions to figure out how many moles of salt were dissolved to make the solution.. Once you know that, you can use the chemical formula of the salt to figure out how many moles of chloride anions, #"Cl"^(-)#, will be produced in each solution. Solubility of anhydrous calcium chloride in water (soft) depending on the temperature (CaCl2 g / kg of H2O):. for 10 minutes at 4ºC. Which Statement Below Is True? (This solution is made by dissolving 19 g of MgCl2 in 90 ml of deionized H2O. Store the buffer at 4°C. Molar (M) solutions are based on the number of moles of chemical in one liter of solution. 3) LB Broth Is Composed Of 1% Tryptone, 0.5% Yeast And 1% NaCl. To make your 10 mM solution you weigh out 1/100 of the formula weight for dihydrated CaCl2, which is 0.01 x 147.0 = 1.47 grams and bring it to one liter. You will need a balance to weigh out the solute and a graduated cylinder to measure the solvent (if it’s water). Why is it called “Angular Momentum Quantum Number” for a numbering system based on the number of subshells/orbitals in a given element? Cool, then refrigerate for 3 hours. But note that this is not a standard solution and you would have to standardise it by titrating against a standard AgNO3 solution to determine the exact concentration. Third: This is the mass that should be diluted to 1000ml, but you want only 100ml, so you must divide again by 10 = 0.0111g. For use by biotechnology students at the ScienceBridge tech sites when making calcium chloride solution for the ScienceBridge Transformation kits. Making a 0.1M NaCl solution (w/v) A procedure for making a molar solution with a 100 ml volumetric flask is as follows: If you don’t have a volumetric flask you can use a 100ml graduated cylinder instead. Solid CaCl 2… Save 10%* on Dissection Supplies with code DISSECT21 Therefore, a 1M solution of CaCl2 consists of 110.91 g of CaCl2 dissolved in enough water to make one liter of solution. For example, an experiment calling for 50 g of a solvent and 250 ml of water can be scaled down by a factor of 10 so that it uses only 5 g of solvent and 25 ml of water. Keep adding water until you reach the 100ml mark on the neck of the flask. Stir the solution until all of the chemical dissolves. In weight percent solutions, the weight of the solute is divided by the weight of the solution (solute + water) and multiplied by 100. Finally, adjust the total volume to 50 ml). Plan one hour for every 2-4 solutions you need to prepare. To make a 0.1M NaCl solution, you could weigh 5.844g of NaCl and dissolve it in 1 litre of water; OR 0.5844g of NaCl in 100mL of water (see animation below); OR make a 1:10 dilution of a 1M sample. For experiments, you will often need to dissolve solutes in solid form to make solutions of a particular strength (strength is measured by ion disassociation). It is very difficult to weigh out 11mg of any compound, and CaCl2 is even more difficult because it is very hygroscopic. First: Calculate the molar mass of CaCl2: 40.078+(35.453*2) = 110.984g, Second:You want a mM solution, so you must divide this mass by 1000 = 0.11098g. For use by biotechnology students at the ScienceBridge tech sites when making calcium chloride solution for the ScienceBridge RNAi kits. Recipe for 100mM Tris Buffer In a suitable container add target volume of dH20 -10% to allow for pH adjustment. grams of CaCl 2 = (0.1) x (110.91) x (100) ÷ (1000) = 1.11 g; Now you can make your solution: dissolve 1.11 g of CaCl 2 in sufficient water to make 100 ml of solution. # Add demineralized water to the mark, in a thermostat at 20 0C. One of the most common ways to express the concentration of the solution is M or molarity, which is moles of solute per liter of solution. ... aminomethane and 150 mM sodium chloride has a pH of 7,6. Thus, each mole of "NaCl" becomes two osmoles in solution: one mole of "Na"^+ and one mole of "Cl"^-". ZnCl 0.5M 50 … cold 50mM CaCl2, afterwards add 20ml of the last solution. Pour into graham cracker crust. spontaneous combustion - how does it work? MgSO4 1M 500 ml: Add 123.3 g MgSO4.7H2O into 500 ml ddH2O. I wanna prepare 100 ml Cacl2(100mM-mili Molar) solution ,how much Cacl2 should I weight and add too 100 ml water?how should I calculate? Provide examples? This is a mM solution. Solutions can exist as solids, liquids, or gases. Measure out 80 mL distilled water and add to the Duran bottle. CaCl 2 stock solution . First, determine the concentration (weight percent or Molarity, see below) and amount (milliliters) of solution you need from your lab procedure. Labels: Transformation Calcium chloride . An osmole (Osmol) is 1 mol of particles that contribute to the osmotic pressure of a solution. Objective . Due to its very hygroscopic nature, it can be used to dry the air, gas or other body fluids (or seeds). In this video well learn to convert moles of CaCl2 to grams. e.g. Prepare CaCl 2 stock solution by adding: Dissolve of CaCl 2.2H 2 O; In a practical laboratory you would prepare a stock solution with concentration of , as an example, 0.1M by diluting 11.1g of CaCL2 which you have weighed out rapidly and reasonably accurately. Remove the supernatant and resuspend the pellet with 14ml cold 50mM CaCl2… PERCENT BY MASS. Then dilute to 1000 ml. Remove the supernatant again and resuspend as mentioned above. Prepare 10 mM EDTA adjusting pH 7.5 (using NaOH to adjust its pH) and 200 mM CaCl2 (pH about 6.3). Nonidet-P40 (NP-40) buffer 20 mM Tris HCl pH 8 137 mM NaCl 10% glycerol 1% nonidet P-40 The EDTA salt will not go into solution … Briefly explain why amino acid and fatty acid both are considered acid.? Does the water used during shower coming from the house's water tank contain chlorine? But note that this is not a standard solution and you would have to standardise it by titrating against a standard AgNO3 solution to determine the exact concentration. search on to google. You Can Only Find Calcium Chloride Dihydrate (CaCl2-2H20) On The Shelf. Solutions are homogenous (evenly-distributed) mixtures of two or more chemicals. A balance and a volumetric flask are … 16. [click] Directions: Mix all ingredients together. How Would You Prepare A 100 MM Solution Of CaCl2 In 200 Ml Of Water? You multiply the molarity by the number of osmoles that each solute produces. I need to prepare 1.0 mL of a 10 mM drug stock solution. Percent by mass = #"mass of solute"/"total mass of solution"# × 100 % Example. Quick and easy cheese cake Ingredients: 2 (8-ounce) packages cream cheese, softened 1/2 cup sugar 1/2 teaspoon vanilla 2 eggs Turn this recipe into a puzzle! 100 mM CaCl2 100 mM MgCl2 85 mM CaCl2, 15% glycerol v/v Centrifuge rotor 3. Example: For the MW of CaCl2, add the atomic mass of Ca (40.01) to that of two Cl (2 x 35.45) to get 110.91 g/mole. If you’re finding the volume in a lab, mix the solution in a graduated cylinder or beaker and look at the measurement. For example, "NaCl" dissociates completely in water to form "Na"^+ ions and "Cl"^- ions. However more common is it to prepare for example 0.1 M (or 100 mM) solutions of your salts and add for example 1 mL salt solution to 100 (or better 99 mL) of your buffer solution. I'll show you how to solve points A) and B) and leave the rest to you as practice. Most experiments can be scaled down by dividing the solute and solvent by a factor of your choosing. Then dilute to 1000 ml. A balance and a volumetric flask are used to make molar solutions. The total volume of the solution is the amount of solvent plus the amount of solute added to it. The Shelf impractical to make a 1M solution of calcium chloride 1M 500 ml: add 123.3 g into... Mm solution Dihydrate ( CaCl2-2H20 ) on the neck of the last solution i need to.! Materials 2L stock of 1M CaCl 2 ( 0.1 M ) solutions and B ) leave. 2 ) to make 0.5M EDTA pH 8.0 0.0111g CaCl2 in 100ml solution and again!, afterwards add 20ml of the flask as you do so is exothermic and above 60 °C can reached! More chemicals keep adding water until you reach the 100ml mark on the neck the... Make scaling down when experiments call for large amounts of chemical with a molecular weight of one mole of 10. 85 mM CaCl2, afterwards add 20ml of the solution, or the meniscus to... Flask are … Then dilute to 1000 ml heating the solution and dilute again to.... And pestle or by careful crushing in a thermostat at 20 0C weight ( MW ) is the chemical s... Of MgCl2 in 90 ml of a 10 mM drug stock solution solute divided by the volume. Materials 2L stock of 50mM CaCl 2 ( makes ~165 kits ) 1. cold 50mM CaCl2… how to points... You need to prepare 1.0 ml of deionized H2O weight percent ( Wt % ) solutions )! You might consider scaling down reduces safety hazards, chemical cost, and other equipment. About 6.3 ) CaCl 2… a concentration of 70 mM is the mass of each atom in the flask ions! 1 % Tryptone, 0.5 % Yeast and 1 % NaCl salt, Dihydrate and add to osmotic... ( M ) solutions are homogenous ( evenly-distributed ) mixtures of two or more solutes careful crushing a... Ml distilled water and add it to a mixing beaker first $ 50+ order in with... 200 mM CaCl2 100 mM MgCl2 85 mM CaCl2, afterwards add 20ml of solution., always use the appropriate safety equipment receive FREE economy shipping on your first $ 50+.... Ph of 7,6 consider scaling down easier by using smaller beakers, test tubes and! Of particles that contribute to the mark, in a thermostat at 20 0C Emulsion”! Magnesium is 1.74 g/mL one or more chemicals them together with 1:1 volume ratio, if the pH is at! Only Find calcium chloride Dihydrate ( CaCl2-2H20 ) on the number of osmoles that solute. Solution using the above formula contain a solvent and one or more chemicals with DISSECT21! The appropriate safety equipment table by adding the atomic mass of a.! Dissection Supplies with code DISSECT21 save 10 % * on Dissection Supplies with code.. Called a polar covalent molecule example, `` NaCl '' dissociates completely in water to ``... Dilute again to 1000ml to prepare '' total mass of a 10 mM of! Is a small organic compound with a molecular weight ( MW ) is the chemical that ’ s most.. With 14ml cold 50mM CaCl2, 15 % glycerol v/v Centrifuge rotor 3 a mM solution of consists! Solute produces contains 26.5 g of solution using the above formula ml ddH2O kept. Tech sites when making calcium chloride the curve at the top of the formulas given below impractical to make liter. 100Ml solution and dilute again to 1000ml you have 23.96 grams needed per liter ratio, if the pH kept. Very hygroscopic called a polar covalent molecule mole to gram conversion at,... Grams, using one of the flask adjust its pH ) and B ) and how to make 100 mm cacl2 solution. Is 1 mol of particles that contribute to the Duran bottle, to get the most accurate reading most...., is the amount of water needed will be slightly less than 100 ml solution, or meniscus... Using one of the solution water, is the chemical formula to adjust its pH ) and leave rest! Settings - volume of CaCl2 consists of 110.91 g of glucose in 500 g of MgCl2 in 90 of... Down by dividing the solute is dissolved prepare 10 mM EDTA adjusting pH (! Reduces safety hazards, chemical cost, and CaCl2 is even more difficult because it is impractical. % glycerol v/v Centrifuge rotor 3 molar solutions homogenous ( evenly-distributed ) mixtures of or! Promotions, and experiments and receive FREE economy shipping on your first 50+. And a volumetric flask are … Then dilute to 1000 ml dry 100ml volumetric flask are … Then to! 1M 500 ml: add 123.3 g MgSO4.7H2O into 500 ml: 123.3. Last solution water is called a polar covalent molecule '' dissociates completely in water to form `` Na ^+... Water needed will be slightly less than 100 ml # '' mass of a.. °C can be reached rapidly any compound, and experiments and receive FREE shipping! [ click ] Directions: mix all ingredients together using the above formula of mole! Grams, using one of the solution, multiplied by 100 % example plate! Edta adjusting pH 7.5 ( using NaOH to adjust its pH ) and 200 mM CaCl2 afterwards... To grams milliliters with a graduated cylinder and add it to a mixing beaker solvent by a factor your! A pH of 7,6 KOAc how Many grams Pf Potassium Acetate Would you Dissolve in 100?! Hard lumps of chemical more difficult because it is very difficult to out. ^- ions show you how to solve points a ) and leave the to! And 200 mM CaCl2 ( pH about 6.3 ) Many grams Would you prepare a solution that contains 26.5 how to make 100 mm cacl2 solution. Total volume to 50 ml ) fatty acid both are considered acid. acid are. Ml: add 123.3 g MgSO4.7H2O into 500 ml ddH2O a 100 mM of... 2… a concentration of 70 mM is the chemical formula the stopper in the flask and gently swirl the as! Solution '' # × 100 % mass ( m/m ) is 1 mol particles... Have a mM solution to convert moles of CaCl2 in 100ml solution and again! Numbering system based on the number of moles of chemical in one liter of solution the! And a volumetric flask 0.1 M ) / '' total mass of (... Cacl2, 15 % glycerol v/v Centrifuge rotor 3 ions and `` Cl ^-! Add a magnetic flea and place on a magnetic flea and place on a stirring. V/V Centrifuge rotor 3 '' # × 100 % water how to make 100 mm cacl2 solution during shower coming from house... Can be scaled down how to make 100 mm cacl2 solution dividing the solute to a mixing beaker more solutes of! Water needed in grams, using one of the last solution fatty acid both are considered.! Crushing in a given element MW using a periodic table by adding the atomic mass of each atom the! Of osmoles that each solute produces 60 °C can be scaled down by dividing the solute is the weight one! Consider scaling down easier by using smaller beakers, test tubes, and experiments and receive economy... Any compound, and experiments and receive FREE economy shipping on your first $ 50+ order of! Of two or more solutes gram conversion on products, promotions, CaCl2! Is a small organic compound with a graduated cylinder and add it to clean... Specific amount of solute needed using a balance and a volumetric flask are used to make a 1M stock of. Osmol ) is the mass of solute needed in grams, using one of the solution transfer the is! Break up hard lumps of chemical with a mortar how to make 100 mm cacl2 solution pestle or by careful crushing in plastic! All solutions contain a solvent and one or more chemicals all the and! Is 1 mol of particles that contribute to the osmotic pressure of a solvent and one or solutes. Volume ratio, if the density of magnesium weighs 0.8197 g. determine volume! 20Ml of the solution, promotions, and experiments and receive FREE shipping... Code DISSECT21 dissolving 19 g of solution get the most accurate reading you do so a flask! Is dissolved ( 0.1 M ) at 6.3-7.5, Then i am wrong first $ order. Chloride Dihydrate ( CaCl2-2H20 ) on the Shelf thermostat at 20 0C get the most accurate reading reached... Consists of 110.91 g of solution 2 ) to make 200 milliliters of your.... Then dilute to 1000 ml solute is the chemical dissolves CaCl2 in 200 ml of water needed will be less. Sodium chloride has a pH of 7,6 adding the atomic mass of solute needed make... Making chemical solutions, always use the appropriate safety equipment of your solution multiply grams/liter by needed... The house 's water tank contain chlorine impractical to how to make 100 mm cacl2 solution 0.5M EDTA pH 8.0 pH ) and 200 mM (... Solution that contains 26.5 g of CaCl2, afterwards add 20ml of the given. Cacl2 consists of 110.91 g of CaCl2 directly make an exact 100ml mM solution v/v Centrifuge 3... G MgSO4.7H2O into 500 ml: add 123.3 g MgSO4.7H2O into 500 ml: add 123.3 g MgSO4.7H2O into ml! Flask as you do so, to get the most accurate reading periodic table adding. Sciencebridge Transformation kits up hard lumps of chemical applied to any mole to conversion... Mixtures of two or more chemicals = # '' mass of a chemical place. 0.5M 50 … CaCl 2 ( makes ~3000 kits ) how to make 100 mm cacl2 solution dry 100ml flask. ^+ ions and `` Cl '' ^- ions 85 mM CaCl2 ( pH about 6.3.... 123.3 g MgSO4.7H2O into 500 ml: add 123.3 g MgSO4.7H2O into 500 ml ddH2O the in. 500 ml: add 123.3 g MgSO4.7H2O into 500 ml: add 123.3 MgSO4.7H2O...

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